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21.
Masamitsu Kosaki Masayuki Nagao Yukio Mizuno Noriyuki Shimizu Kenji Horii 《Electrical Engineering in Japan》1991,111(6):67-75
A superconducting power cable is one of the promising ways of underground transmission of huge electric power in the future. The authors have long proposed the idea of the extruded polymer insulation for superconducting cables. The prominent features of the design are to exploit the excellent electrical properties of polymer in the cryogenic temperatures and to separate the helium coolant from the electrical insulation. Although the extruded cross-linked polyethylene cable has proved ability at the liquid nitrogen temperature, the cable insulation cracked due to mechanical stress during cooling to the liquid helium temperature. To overcome this problem, ethylene propylene rubber (EPR) was selected as a new insulating material considering the good results of mechanical and electrical tests of EPR samples at cryogenic temperatures. An extruded EPR insulated superconducting cable 15 m in length was fabricated and a cooling test down to the liquid helium temperature and a voltage test at the liquid helium temperature were carried out with fair success. This is a breakthrough in terms of the electrical insulation design of cryogenic cables. 相似文献
22.
Mitsuyoshi Ishikawa Shohei Yamamura Yuzuru Takamura Koji Sode Eiichi Tamiya Masamitsu Tomiyama 《International Journal of Hydrogen Energy》2006
The development of a compact high-density microbial reactor for hydrogen production is described with possible implications to use as a portable bio-fuel cell system. To construct the compact bioreactor, mainly, the cell density and immobilization methods were optimized in this paper. The encapsulation of hydrogen producing bacterium, Escherichia coli strain MC13-4, in alginate gel beads provided approximately three-fold increase in hydrogen production in comparison with the free cell suspension. The immobilized cells (cell density; O.D. 100) and 500 mM glucose solution were packed into a 20 mL glass bottle that was connected to the fuel cell. This system has generated electricity of over 20 mW for 20 min. 相似文献
23.
24.
Tetsuroh Tokoro Kazuyuki Tohyama Masayuki Nagao Masamitsu Kosaki 《Electrical Engineering in Japan》1992,112(6):10-19
Nonpolar polymers such as polyethylene or polypropylene are widely used as insulation materials, often under conditions involving strong electric fields and high temperatures. Under these conditions, the conduction loss due to DC-like carrier transport may give rise to a high-field AC dissipation factor (tan δ). This paper describes the high-field dielectric properties of polyethylene films at frequencies from 50 to 400 Hz and temperatures from room temperature to 100°C, as measured with a new type electrode design developed by the authors. In the high-temperature region, the AC dissipation current waveform at 50 Hz was also measured. At room temperature, the electric-field dependence of tan δ is almost unaffected by the frequency of the applied field. But at high temperatures and strong electric fields, tan δ tends to have a large field dependence and to be almost inversely proportional to the frequency. Thus the AC conductivity becomes independent of the frequency of the applied field in the region of strong fields and high temperatures. A theoretical analysis shows that the high-field dielectric loss is governed by two processes, namely, the dielectric relaxation loss inherent to the AC field and the DC-like carrier transport loss that becomes prominent in the high-temperature region, where AC dissipation current waveform becomes significantly nonsinusoidal. 相似文献
25.
This paper deals with the non-repetitive run-out (NRRO) of an angular contact ball bearing for machine tools. First, a measuring system for the rotational accuracy of the bearing was developed. Then, the following results were obtained using the developed measuring system.
- (1)
- The NRRO of ball cage rotation increased with rotational speed.
- (2)
- The influence of form errors in the inner and outer rings on NRRO was small.
- (3)
- The influences of form errors in the balls of the bearing and the diameter difference of balls on NRRO were significant.
26.
This paper deals with the prototype of a new pivot bearing having two degrees of freedom. The idea of the pivot bearing is based on a continuous velocity joint (CVJ). The experimental axial stiffness and contact pressure are compared with those determined by theoretical analysis. Then, it is confirmed that the pivot bearing swings smoothly with a range of ±25°. Furthermore, the stiffness of the bearing increases as the swinging angle becomes larger. Therefore, this newly developed pivot bearing may be applied to a parallel mechanism, a joint of robot and so on. 相似文献
27.
Chemical Approach to Biological Safety: Molecular‐Level Control of an Integrated Zinc Finger Nuclease 下载免费PDF全文
Dr. Masamitsu N. Asaka Dr. Kohsuke Kato Zita Fábián Prof. Dr. Chris Oostenbrink Dr. Hans E. M. Christensen Prof. Dr. Kyosuke Nagata Dr. Béla Gyurcsik 《Chembiochem : a European journal of chemical biology》2018,19(1):66-75
Application of artificial nucleases (ANs) in genome editing is still hindered by their cytotoxicity related to off‐target cleavages. This problem can be targeted by regulation of the nuclease domain. Here, we provide an experimental survey of computationally designed integrated zinc finger nucleases, constructed by linking the inactivated catalytic centre and the allosteric activator sequence of the colicin E7 nuclease domain to the two opposite termini of a zinc finger array. DNA specificity and metal binding were confirmed by electrophoretic mobility shift assays, synchrotron radiation circular dichroism spectroscopy, and nano‐electrospray ionisation mass spectrometry. In situ intramolecular activation of the nuclease domain was observed, resulting in specific cleavage of DNA with moderate activity. This study represents a new approach to AN design through integrated nucleases consisting of three (regulator, DNA‐binding, and nuclease) units, rather than simple chimera. The optimisation of such ANs could lead to safe gene editing enzymes. 相似文献
28.
Ichikawa T Bando M Kondo Y Nakahara M 《Philosophical transactions. Series A, Mathematical, physical, and engineering sciences》2012,370(1976):4671-4689
Unitary operations acting on a quantum system must be robust against systematic errors in control parameters for reliable quantum computing. Composite pulse technique in nuclear magnetic resonance realizes such a robust operation by employing a sequence of possibly poor-quality pulses. In this study, we demonstrate that two kinds of composite pulses-one compensates for a pulse length error in a one-qubit system and the other compensates for a J-coupling error in a two-qubit system-have a vanishing dynamical phase and thereby can be seen as geometric quantum gates, which implement unitary gates by the holonomy associated with dynamics of cyclic vectors defined in the text. 相似文献
29.
Ryota Hosomi Kenji Fukunaga Midori Fukao Munehiro Yoshida Hirofumi Arai Seiji Kanda Toshimasa Nishiyama Teruyuki Kanada 《Food science and biotechnology》2012,21(5):1335-1342
This study evaluated phospholipids (PLs) containing n-3 polyunsaturated fatty acids (n-3 PUFAs) for their specific inherent effects and effects due to a combination of the presence of glycerophosphate structure and n-3 PUFAs on cholesterol metabolism in rats. Rats were fed a diet of AIN-93G containing soybean oil (SO, 7%), SO (5.8%)+fish oil (1.2%), SO (5.2%)+soybean PLs (1.8%), SO (5.2%)+PLs containing n-3 PUFAs (1.8%), and SO (4.0%)+fish oil (1.2%)+soybean PLs (1.8%). Diets with PLs containing n-3 PUFAs, and soybean PLs in combination with fish oil, resulted in decreased serum and liver cholesterol levels through enhancement of fecal cholesterol excretion and suppression of liver sterol regulatory element binding protein-2 mRNA expression compared with the diet containing soybean oil alone. This study shows that soybean PLs with added triacylglycerol that included n-3 PUFAs have the same effects on cholesterol metabolism as PLs containing n-3 PUFAs, and that these could be of benefit to people. 相似文献
30.
We describe new heterologous modules for PCR-based gene targeting in the fission yeast Schizosaccharomyces pombe. Two bacterial genes, hph and nat, which display dominant drug-resistance phenotypes, are used as new selectable markers in these modules. Both genes have been used successfully in the budding yeast Saccharomyces cerevisiae, in which hph confers resistance to hygromycin B, while nat confers nourseothricin resistance (Goldstein and McCusker, 1999). Vector modules for gene disruption and C-terminal tagging with 3HA, 13Myc and GFP(S65T) are constructed using previously constructed pFA6a-MX6-derived plasmids (B?hler et al., 1998; Wach et al., 1997). In combination with the existing systems that are based upon the G418-resistance gene (kan), triple gene deletions or tags could be constructed. In addition a vector for one-step integration of a monomeric RFP (mRFP) to the C-terminus of proteins of interest is developed. Finally, oligonucleotides that allow a simple marker switch from kan to hph or nat, and vice versa, are described. The new constructs developed here should facilitate post-genomic molecular analysis of protein functions in fission yeast. 相似文献